|
KMID : 0364820150510030263
|
|
Korean Journal of Microbiology
2015 Volume.51 No. 3 p.263 ~ p.270
|
|
|
Characterization of two β-mannanases from Cellulosimicrobium sp. YB-43
|
|
Yoon Ki-Hong
|
|
|
Abstract
|
|
|
|
A bacterial strain producing extracellular mannanases was isolated from soil of chestnut tree farm located in Gongju city of Korea by enrichment culture using Avicel as a carbon source. 16S rDNA sequence of the isolate YB-43 was highly homologous to those of genus Cellulosimicrobium strains with sequence similarities of above 99.6%. Mannanase productivity was significantly increased when the Cellulosimicrobium sp. YB-43 was grown in the presence of locust bean gum (LBG) or konjac. The mannanases were partially purified to be mannanase A (ManA) and mannanase C (ManC) by DEAE-Sepharose column and Q-Sepharose column chromatography from the culture filtrate of Cellulosimicrobium sp. YB-43 grown in LB medium supplemented with 0.7% LBG for 24 h. The partially purified ManA showed the highest activity at 55°C and pH 6.5, while ManC activity was optimal at 65°C and pH 7.5. ManA was stable up to 40°C for 1 h, but ManC activity decreased significantly even after 1 h at 20°C. ManA and ManC showed difference from each other according to their substrate specificities and predominant products resulting from the mannanase hydrolysis for mannooligosaccharides. As a result, Cellulosimicrobium sp. YB-43 was found to produce two different kinds of mannanases.
|
|
|
KEYWORD
|
|
|
Cellulosimicrobium sp. YB-43, mannanases, productivity, property
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|